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Image Search Results
Journal: Frontiers in Genetics
Article Title: Comprehensive Analysis of the Tumor Microenvironment and Ferroptosis-Related Genes Predict Prognosis with Ovarian Cancer
doi: 10.3389/fgene.2021.774400
Figure Lengend Snippet: Analyses of survival-related FRGs in OC. (A) Ferroptosis pivot genes, namely, SLC7A5, ACSL4, XCT, GPX4, and β-actin protein expression in platinum-resistant tissues (R1-R3) and platinum-sensitive tissues (S1-S3). (B) SLC7A5, ACSL4, XCT, GPX4, and β-actin protein expression in ovarian cell lines (OVCAR3 and SKOV3) and platinum-resistant OC cell lines (OVCAR3-CDDP and SKOV3-CDDP). (C) Cell viability assays under cisplatin treatment; SKOV3/CDDP showed higher cisplatin tolerance, representing the resistance phenotype. Cells were treated with cisplatin (2.2 μg/ml for SKOV3 and 6.9 μg/ml for SKOV3-CDDP) in the absence or presence of erastin (10 μM), RSL3 (0.1 μM), and NAC (1 mM) for 24 h and cell viability. (D) Glutathione (GSH) (E) and lipid peroxidation (MDA) assays (F) were performed. (G) XCT, GPX4, and β-actin protein expression in SKOV3 (i) and SKOV3-CDDP (ii) under the abovementioned treatment. Error bars represent the standard deviation (s.d.) of triplicate measurements. * p < 0.05 and ** p < 0.01.
Article Snippet: OC cell lines, namely,
Techniques: Expressing, Standard Deviation